Uji Kualitas Mikrobiologis Air Di Pondok Pesantren Sukahideng Tasikmalaya Menggunakan Metode Membran Filter

Maulana, Fikri (2025) Uji Kualitas Mikrobiologis Air Di Pondok Pesantren Sukahideng Tasikmalaya Menggunakan Metode Membran Filter. Diploma thesis, UNIVERSITAS BTH TASIKMLAYA.

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Abstract

ABSTRAK Uji Kualitas Mikrobiologis Air Di Pondok Pesantren Sukahideng Kabupaten Tasikmalaya Menggunakan Metode Membran Filter Fikri Maulana D-III Analis Kesehatan/TLM, Universitas Bakti Tunas Husada, Tasikmalaya Abstrak Penelitian ini bertujuan mengevaluasi kualitas mikrobiologis air sumur dan depot air minum di sekitar Pondok Pesantren Sukahideng, Kabupaten Tasikmalaya, melalui identifikasi bakteri Escherichia coli menggunakan metode membran filter. Kontaminasi mikrobiologis air merupakan ancaman serius bagi kesehatan santri. Metode membran filter dipilih karena memiliki sensitivitas tinggi dalam mendeteksi Escherichia coli sebagai indikator kontaminasi feses. Penelitian menggunakan metode deskriptif dengan pendekatan cross-sectional dan teknik purposive sampling pada 5 titik (2 depot dan 3 sumur). Analisis dilakukan di laboratorium dengan media selektif dan identifikasi morfologi koloni, serta dibandingkan dengan standar Permenkes No. 2/MENKES/PER/I/2023. Hasil menunjukkan 80% sampel tidak memenuhi standar kualitas air. Kontaminasi Escherichia coli ditemukan di Depot P1 (2 CFU/100ml), Sumur A (5), Sumur B (13), dan Sumur C (16), sementara Depot P2 negatif (0). Kandungan bakteri Coliform juga tinggi di sumur, melebihi 100 CFU/100ml. Kata kunci: Escherichia coli, kualitas air, membran filter, pondok pesantren, mikrobiologi air, kontaminasi feses. Abtract This study aims to evaluate the microbiological quality of well water and drinking water depots around the Sukahideng Islamic Boarding School, Tasikmalaya Regency, through the identification of Escherichia coli bacteria using the membrane filter method. Microbiological contamination of water is a serious threat to the health of students. The membrane filter method was chosen because it has high sensitivity in detecting Escherichia colias an indicator of fecal contamination. The study used a descriptive method with a cross-sectional approach and purposive sampling technique at 5 points (2 depots and 3 wells). Analysis was carried out in the laboratory with selective media and identification of colony morphology, and compared with the standards of the Minister of Health Regulation No. 2/MENKES/PER/I/2023. The results showed that 80% of samples did not meet water quality standards. Escherichia coli contamination was found in Depot P1 (2 CFU/100ml), Well A (5), Well B (13), and Well C (16), while Depot P2 was negative (0). The Coliform bacteria content was also high in the wells, exceeding 100 CFU/100ml. Keywords: Escherichia coli, water quality, membrane filter, Islamic boarding school, water microbiology, fecal contamination

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