UJI DAYA HAMBAT EKSTRAK ETANOL 70% JAMUR LINGZHI (Ganoderma lucidum) TERHADAP PERTUMBUHAN JAMUR Pityrosporum ovale SECARA IN VITRO

HERMALIA, NERSA (2026) UJI DAYA HAMBAT EKSTRAK ETANOL 70% JAMUR LINGZHI (Ganoderma lucidum) TERHADAP PERTUMBUHAN JAMUR Pityrosporum ovale SECARA IN VITRO. Diploma thesis, UNIVERSITAS BAKTI TUNAS HUSADA TASIKMALAYA.

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Abstract

Pityrosporum ovale merupakan jamur bersel tunggal (yeast) yang termasuk ke dalam genus Malassezia dan dikenal sebagai salah satu mikroorganisme yang berperan dalam terjadinya pityriasis atau ketombe. Jamur Ganoderma lucidum mengandung berbagai senyawa bioaktif, seperti flavonoid, triterpenoid, alkaloid, saponin, dan tanin, yang dilaporkan memiliki aktivitas antijamur. Penelitian ini bertujuan untuk mengevaluasi kemampuan ekstrak etanol 70% Ganoderma lucidum dalam menghambat pertumbuhan Pityrosporum ovale pada variasi konsentrasi 10% hingga 100%. Penelitian menggunakan metode eksperimen dengan teknik difusi cakram (Kirby-Bauer). Hasil penelitian menunjukkan bahwa ekstrak etanol 70% Ganoderma lucidum mampu membentuk zona hambat dengan diameter berkisar antara 6,10 mm hingga 7,57 mm. Diameter zona hambat terbesar diperoleh pada konsentrasi 100%, yaitu sebesar 7,57 mm yang termasuk dalam kategori daya hambat sedang. Sebagai pembanding, kontrol positif menggunakan nystatin menghasilkan diameter zona hambat sebesar 10,1 mm dengan kategori sedang, sedangkan kontrol negatif berupa akuades steril tidak menunjukkan adanya zona hambat. Kata Kunci: Ganoderma lucidum, Pityrosporum ovale, Antijamur. Abstract Pityrosporum ovale is a unicellular yeast belonging to the genus Malassezia and is recognized as one of the microorganisms associated with the development of pityriasis (dandruff). Ganoderma lucidum contains various bioactive compounds, including flavonoids, triterpenoids, alkaloids, saponins, and tannins, which have been reported to possess antifungal properties. This study aimed to evaluate the inhibitory activity of 70% ethanol extract of Ganoderma lucidum against the growth of Pityrosporum ovale at concentrations ranging from 10% to 100%. This experimental study employed the Kirby–Bauer disk diffusion method. The results showed that the 70% ethanol extract of Ganoderma lucidum produced inhibition zones ranging from 6.10 mm to 7.57 mm. The largest inhibition zone was observed at the 100% concentration, measuring 7.57 mm, which was categorized as moderate antifungal activity. Nystatin was used as the positive control and produced a moderate inhibition zone with a diameter of 10.1 mm, while sterile distilled water served as the negative control and showed no inhibitory effect. Keywords: Ganoderma lucidum fungus, Pityrosporum ovale, Antifungal.

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