UJI AKTIVITAS NANOKURKUMIN TERHADAP PENGHAMBATAN ENZIM α-GLUKOSIDASE SECARA INVITRO

BUDIANI, SHAFA ANINDYA (2022) UJI AKTIVITAS NANOKURKUMIN TERHADAP PENGHAMBATAN ENZIM α-GLUKOSIDASE SECARA INVITRO. Sarjana thesis, UNIVERSITAS BTH TASIKMALAYA.

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Abstract

Kurkumin diklasifikasikan ke dalam BCS (Biopharmaceutical Classification System) kelas IV berdasarkan kelarutan dalam air yang buruk (4,375 μg/mL) dan bioavailabilitas yang rendah (1.498 ± 0.402 μg jam/mL) (Shelma & Sharma, 2013). Oleh karena itu, diperlukan teknik untuk meningkatkan bioavailabilitas dalam kurkumin salah satunya yaitu nanopartikel. Penelitian ini dilakukan untuk mengetahui inhibisi dan nilai IC50 terhadap aktivitas penghambatan enzim α-glukosidase secara in vitro. Hasil dari nanopartikel kurkumin diuji aktivitas inhibisi terhadap enzim α-glukosidase menggunakan spektrofotometer Uv-Visible pada λ=305 nm dan dihitung persen (%) inhibisi serta nilai IC50 nya. Uji aktivitas nanokurkumin terhadap penghambatan enzim α-glukosidase secara in vitro menunjukkan nilai IC50 3,95 μg/mL sedangkan akarbosa menunjukkan nilai IC50 akarbosa yaitu 4,11 μg/mL. Aktivitas inhibisi nanokurkumin terhadap enzim α-glukosidase menunjukan nilai IC50 yang lebih baik dibandingkan dengan akarbosa dan termasuk ke dalam kategori sangat aktif. Kata kunci: kurkumin, enzim α-glukosidase, nanoteknologi. Curcumin is classified into BCS (Biopharmaceutical Classification System) class IV based on its poor water solubility (4.375 g/mL) and low bioavailability (1,498 ± 0.402 g hour/mL) (Shelma & Sharma, 2013). Therefore, a technique is needed to increase the bioavailability of curcumin, one of which is nanoparticles. This research was conducted to determine the inhibition and IC50 of the inhibitory activity of the α-glucosidase enzyme in vitro. The results of the curcumin nanoparticles were tested for the inhibitory activity of the α-glucosidase enzyme using a Uv-Visible at λ =305 nm and the percent (%) inhibition and IC50 value were. Against the inhibition of α-glucosidase enzyme vitro showed an nanocurcumin 3,95 μg/mL while acarbose showed an IC50 value of in acarbose that was 4,11 μg/mL. Against the inhibition α-glucosidase enzyme showed nanocurcumin a better IC50 value than acarbose, and included in the very active category. Keywords: nanocurcumin, α-glucosidase enzyme, nanotechnology

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