PENGARUH AIR PERASAN JERUK NIPIS (Citrus aurantiifolia S) TERHADAP PERTUMBUHAN Pityrosporum Ovale SECARA IN VITRO

LESTARI, SASKIA DWI (2022) PENGARUH AIR PERASAN JERUK NIPIS (Citrus aurantiifolia S) TERHADAP PERTUMBUHAN Pityrosporum Ovale SECARA IN VITRO. Diploma thesis, UNIVERSITAS BTH TASIKMALAYA.

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Abstract

ABSTRAK Pengaruh Air Perasan Jeruk Nipis (Citrus aurantifolia S.) Terhadap Pertumbuhan Pityrosporum ovale Secara In Vitro Saskia Dwi Lestari, Rochmanah Suhartati M.Si, Khusnul M.Si Program Studi DIII Teknologi Laboratorium Medik, Univeristas Bakti Tunas Husada Abstrak Dandruff atau pityriasis simpleks adalah istilah umum yang dalam bahasa kedokteran lazimnya disebut dengan ketombe. Ketombe adalah salah satu penyakit yang disebabkan oleh jamur Malassezia sp. salah satunya yaitu Pityrosporum ovale (P. ovale). Tujuan dari penelitian ini yaitu untuk mengetahui pengaruh air perasan jeruk nipis (Citrus aurantifolia S.) terhadap pertumbuahn jamur penyebab ketombe yaitu P. ovale. Penelitian ini bersifat eksperimental dengan Teknik Kirby Bauer, subjek penelitian menggunakan air perasan jeruk nipis (Citrus aurantifolia S.) dan objek penelitian menggunakan isolate murni Pityrosporum ovale pada media SDA dengan kepadatan 0,5 Mc Farland. Hasil penelitian menunjukan adanya pengaruh yang signifikan antara kemampuan air perasan jeruk nipis terhadap jamur P. ovale. Pada perlakuan air perasan jeruk nipis terhadap P. ovale pada konsentrasi 10% memiliki kemampuan daya hambar sebesar (44,3 mm), konsentrasi 20% (36,1 mm), konsentrasi 30% (32,7 mm), konsentrasi 40% (34,1 mm), konsentrasi 50% (34,5 mm), konsentrasi 60% (35,6 mm), konsentrasi 70% (36 mm), konsentrasi 80% (38 mm), konsentrasi 90% (41,2 mm), dan pada konsentrasi 100% (45,7 mm). Kata Kunci : Citrus aurantifolia S., Pityrosporum ovale, daya hambat, Ketokonazole 2%. Abstract Dandruff or pityriasis simplex is a general term that in medical language is usually referred to as dandruff. Dandruff is a disease caused by the fungus Malassezia sp. One of them is Pityrosporum ovale (P. ovale). The purpose of this study was to determine the effect of lime juice (Citrus aurantifolia S.) on the growth of the fungus that causes dandruff, namely P. ovale. This research is experimental using the Kirby Bauer technique, the research subject uses lime juice (Citrus aurantifolia S.) and the research object uses pure isolate Pityrosporum ovale on SDA media with a density of 0.5 Mc Farland. The results showed that there was a significant effect on the ability of lime juice to P. ovale fungus. In the treatment of lime juice on P. ovale at a concentration of 10%, the ability of the taste buds was (44.3 mm), 20% concentration (36.1 mm), 30% concentration (32.7 mm), 40% concentration ( 34.1 mm), 50% concentration (34.5 mm), 60% concentration (35.6 mm), 70% concentration (36 mm), 80% concentration (38 mm), 90% concentration (41.2 mm) ), and at a concentration of 100% (45.7 mm). Keywords: Citrus aurantifolia S., Pityrosporum ovale, inhibitory power, Ketoconazole 2

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